Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: Confocal photomicrographs taken at 60× magnification, seminiferous tubules collected from the corn oil–treated rats utilized for the staining and microscopy, and colored text on the image refers to the immunolabeled entity. (A–D) CD2 cells + are present within the PTMC layer, and their cell shape is irregular; primarily, the CD2 + cells’ shape is determined by the cellular space between the PTMCs. Arrow marks highlight the spot of the empty cellular space between the PTMCs where CD2 + cells localize. (E–G) Arrow marks highlight the spot of the cellular space between the PTMCs where MHC II + PTM φ localizes. Based on the characteristic nuclear morphology, the asterisk symbol points to the nucleus, which is presumed to be a CD2 + cell. (H) Cell-to-cell interaction between an MHC II + PTM φ and CD2 + cell, blue—nuclei. (H′) Masked and surface rendered image visualizing the type of cellular interaction where the membrane of the CD2 + cell is extended or pinched by the PTM φ , nearly half of the CD2 + cell surface was overlapped with PTM φ surface. (I) Another type of cell-to-cell contact. (I) Surface-rendered image shows a clear cell-to-cell contact between MHC II + PTM φ and CD2 + cell, where the membrane of PTM φ overlaps with the boundary of the CD2 + cell membrane. Scale bar: 15 μ m in (A–G).

Article Snippet: MHC II , Novus Biologicals , NBP3-09015.

Techniques: Staining, Microscopy, Immunolabeling, Membrane

Journal: Biology of reproduction

Article Title: Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats

doi: 10.1093/biolre/ioaf162

Figure Lengend Snippet: (A, B) Representative confocal photomicrographs of whole mount seminiferous tubules from rats at 48 h post-exposure, green—PTM φ labeled for MHC II protein, n = 5. (A) Corn oil. (B) MEHP treatment group. (C) Graph presenting the average number of PTM φ per 10 5 area, MEHP-exposed animals showed a significant increase in MHC II + PTM φ s, calculating changes in PTM φ s number with n = 5 rats per treatment group. (D) Representative confocal photomicrograph of whole-mount seminiferous tubules from rats 48 h post-exposure from the corn oil–treated animals (red—CD2 + cells and green—PTM φ labeled for MHC II protein). (E) A representative confocal photomicrograph from the MEHP treatment. (F) Graph presenting the average ± SEM (standard error of the mean) of PTM φ s-CD2 + cells (red) interaction per 10 5 area. The average number of cellular interactions between the PTM φ s and CD2 + cells increased significantly in the MEHP-exposure group when compared to the corn oil–treated group, ( n = 5/group). Scale bar: 15 μ m applies to all the images in this panel.

Article Snippet: MHC II , Novus Biologicals , NBP3-09015.

Techniques: Labeling

Journal: Communications Biology

Article Title: Platelet extracellular vesicles preserve lymphatic endothelial cell integrity and enhance lymphatic vessel function

doi: 10.1038/s42003-024-06675-8

Figure Lengend Snippet: CellVue + human PEVs (1 × 10 7 ) or vehicle control were injected in the dermis of the footpad in wild-type mice. Lymph nodes (LNs) draining the site of injection (green boxes) and LNs located at a distal site (beige boxes) were collected 15 min and 2 h post-injection. Then, they were ( a ) visualized with a fluorescence imaging scanner (IVIS Lumina II) or ( b ) digested with collagenase D to determine the percentage of cells containing EVs. c The concentration of free EVs within the LNs were assessed by flow cytometry using anti-mouse MHCII and anti-human CD62P antibodies. d Two hours post-PEV injection, popliteal lymphatic vessels draining the injection site were also collected, harvested and stained with anti-human CD41a antibody (red) and DAPI (blue) to be imaged with a confocal microscope (white scale bar, 20 µm). The lymphatic contraction capacity was measured in vivo 48 h after the injection of ( e ) PEVs or ( f ) rbEVs in Ldlr -/- , and ( g ) after injection of PEVs in wild-type mice. h The lymph nodes (N-D, beige; D, green) and ( i ) the aorta were also collected at 48 h and visualized with fluorescent imaging. Each point represents a mouse ± SEM. N-D: non-draining; D: draining; LN: lymph nodes; EVs: extracellular vesicles. PEVs: platelet extracellular vesicles. rbEVs: red blood cell extracellular vesicles. Created with Biorender.com .

Article Snippet: To identify the proportion of LN cells positive in PEVs, cells were resuspended in HBSS buffer supplemented with 0.5 M EDTA and 30% bovine serum albumin (BSA) and stained for 30 min at 4 °C with anti-mouse CD45 (FITC, Tonbo Biosciences cat. #350451), anti-mouse MHCII (V450, Tonbo Biosciences cat. #755321) and anti-human CD62P (PE, BioLegends cat. #304905) antibodies (Supplementary Fig. ).

Techniques: Control, Injection, Fluorescence, Imaging, Concentration Assay, Flow Cytometry, Staining, Microscopy, In Vivo